NU 2024-047

SHORT DESCRIPTION

An engineered probiotic that senses intestinal inflammation via calprotectin and locally releases therapeutic antibodies in the gastrointestinal tract only during inflammatory flares.

BACKGROUND

Inflammatory bowel disease (IBD), including Crohn’s disease and ulcerative colitis, is a chronic autoimmune condition marked by recurrent inflammation of the gastrointestinal (GI) tract, requiring long‑term management, which is associated with substantial morbidity, impaired quality of life, increased colorectal cancer risk, and significant healthcare costs. Globally, IBD affects an estimated 4.9 million people, with prevalence rising. While effective therapies exist, they are typically delivered systemically and can cause significant side effects due to prolonged immunosuppression. At the same time, disease monitoring relies on invasive or slow diagnostic methods, which can delay timely intervention during inflammatory flares. There is a critical need for therapeutic approaches that both detect intestinal inflammation noninvasively and deliver treatment locally, only when disease activity is present. 

ABSTRACT
Northwestern researchers have developed an engineered probiotic capable of detecting markers indicative of active inflammation in the GI tract and releasing therapeutic antibodies in response for treatment of IBD. This invention integrates synthetic biology with microbial diagnostics. E. Coli Nissle 1917 (EcN)—a probiotic with established safety in humans—was engineered to produce and selectively release therapeutic antibodies in the GI tract only in the presence of calprotectin, the clinical gold standard biomarker of IBD. In both in vitro settings and a DSS-induced murine colitis model, the probiotic showed high sensitivity and specificity to inflammation. The platform distinguished active IBD from remission by quantifying reporter signals that track with clinical calprotectin levels. For disease management, the engineered probiotic can subsequently release a therapeutic antibody, such as anti-TNF-α.  

APPLICATIONS

• IBD Disease Activity Monitoring: Enables noninvasive tracking of gut inflammation.
• Early Intervention Strategies: Supports timely identification of IBD flares to prevent complications.
• IDB Disease Management: Enables precise, inflammation‑responsive medication delivery within the gastrointestinal tract.

ADVANTAGES

• Noninvasive and rapid: Eliminates the need for colonoscopies while providing fast results.
• Highly sensitive and specific: Reduces systemic immunosuppression from IV infusions by enabling local, calprotectin-induced delivery of antibody therapeutics.
• Cost-effective: Reduces overall diagnostic expenses by streamlining monitoring processes.
• Real-time disease tracking: Supports immediate clinical decisions and proactive patient care.

PUBLICATIONS

• Xia, J. Y.; Hepler, C.; Tran, P.; Waldeck, N. J.; Bass, J.; Prindle, A. Engineered Calprotectin-Sensing Probiotics for IBD Surveillance in Humans Proceedings of the National Academy of Sciences, 2023

CATEGORY/INDUSTRY PIPELINE

Healthcare Devices, Tools & IT; Biomarkers & Biomedical Research Tools; Therapeutics

KEYWORDS

Inflammation, immunology, IBD, calprotectin, engineered probiotics, non-invasive monitoring, biosensor, synthetic biology, gut inflammation, diagnostics, autoimmunity, biologic, cell therapy, therapeutics